CMP-N-Acetylneuraminic Acid Synthetase from Escherichia coli K1 Is a Bifunctional Enzyme
نویسندگان
چکیده
منابع مشابه
Identification of Arg-12 in the active site of Escherichia coli K1 CMP-sialic acid synthetase.
Escherichia coli K1 CMP-sialic acid synthetase catalyses the synthesis of CMP-sialic acid from CTP and sialic acid. The active site of the 418 amino acid E. coli enzyme was localized to its N-terminal half. The bacterial CMP-sialic acid synthetase enzymes have a conserved motif, IAIIPARXXSKGLXXKN, at their N-termini. Several basic residues have been identified at or near the active site of the ...
متن کاملCMP - N - acetylneuraminic a id synthetase of Escherichia coliz high level expression , purification and use in
The gene encoding CIIP-A'-acet\ ' lneuraminic acid (CIIPNeuAc) s1'nthetase (EC 2.7.7.43) in Escherichia coli serot!'pe 07 Kl w'as isolated and overexpressed in E.coli W3ll0. \Iaximum expression of 8-1Ooh of the soluble E.coli protein rras achieved b1'placing the gene rr i th an engineered 5'-terminus and Shine-Dalgarno sequence into a pKK223 rector derivative behind the tac promoter. The overex...
متن کاملSynthesis of N-acetylneuraminic acid and of CMP-N-acetylneuraminic acid in the rat liver cell.
Adult male rats, under starving and normal conditions, were injected intravenously with N-acetyl[3H]mannosamine and after various time intervals the specific radioactivities of free N-acetylneuraminic acid (NeuAc) and CMP-N-acetylneuraminic acid were determined in the liver. The specific radioactivity of free NeuAc was high even within 20s after injection; the maximum was reached between 7 and ...
متن کاملCharacterization of CMP-N-acetylneuraminic acid synthetase of group B streptococci.
The capsular polysaccharide is a critical virulence factor for group B streptococci associated with human infections, yet little is known about capsule biosynthesis. We detected CMP-Neu5Ac synthetase, the enzyme which activates N-acetylneuraminic acid (Neu5Ac, or sialic acid) for transfer to the nascent capsular polysaccharide, in multiple group B streptococcus serotypes, all of which elaborate...
متن کاملCMP-N-acetylneuraminic acid synthetase interacts with fragile X related protein 1
Fragile X mental retardation protein (FMRP), fragile X related 1 protein (FXR1P) and FXR2P are the members of the FMR protein family. These proteins contain two KH domains and a RGG box, which are characteristic of RNA binding proteins. The absence of FMRP, causes fragile X syndrome (FXS), the leading cause of hereditary mental retardation. FXR1P is expressed throughout the body and important f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2004
ISSN: 0021-9258
DOI: 10.1074/jbc.m400143200